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|Microbiologie aplicată (245)|
|Microbiologie industrială. micrologie industrială. Fermentare, industrii de fermentare. Industria băuturilor. Industria stimulenților (380)|
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EFREMOVA, Nadejda; BESHLIU, Alina; CHISELITSA, Natalia; CHISELITSA, Oleg; TOFAN, Elena; LOZAN, Ana; DANILIŞ, Marina. The characteristic of the protein extract obtained from brewer’s yeast. In: Yesterday’s heritage – implications for the development of tomorrow’s sustainable society. Ediția 3, 11-12 februarie 2021, Chişinău. Chișinău, Republica Moldova: 2021, pp. 95-96.
|Yesterday’s heritage – implications for the development of tomorrow’s sustainable society
Ediția 3, 2021
Conferința "Yesterday’s heritage – implications for the development of tomorrow’s sustainable society" |
Chişinău, Moldova, 11-12 februarie 2021
Protein extracts obtained with the use of microorganisms are of interest for application in various fields, such as food, pharmaceutical, cosmetics. This paper presents data on the effect of autolysis on protein content and activity of the enzyme superoxidysmutase. Autolysis was performed using acetic acid and phosphate buffer at various temperature limits for 8 hours. According to the results obtained, the maximum protein content was manifested when using the phos phate buffer solution at a temperature of 45°C. The activity of SOD in extracts varies at the level of 71.9 ± 2.19 - 114.7 ± 0.10 U / mg protein with maximum value in the case where sodium phosphate buffer at temperature 37 served as induction factor of autolysis ° C. Thus, it can be mentioned that phosphate buffer can be used for maximum protein and superoxidismutase extraction. Results contributes to improvement the obtaining purified protein preparations from yeast biomass.