Rolul condiţiilor de cultivare in biosinteza β-glucanilor la Saccharomyces cerevisiae CNMN-Y-20

Usatîi Agafia; Chiseliţa Natalia; Molodoi Elena; Efremova Nadejda; Bejenaru (Fulga) Ludmila; Borisova Tamara

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2020-06-09 00:52

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USATÎI, Agafia, CHISELIŢA, Natalia, MOLODOI, Elena, EFREMOVA, Nadejda, BEJENARU (FULGA), Ludmila, BORISOVA, Tamara. Rolul condiţiilor de cultivare in biosinteza β-glucanilor la Saccharomyces cerevisiae CNMN-Y-20. In: Buletinul Academiei de Ştiinţe a Moldovei. Ştiinţele vieţii, 2013, nr. 2(320), pp. 116-125. ISSN 1857-064X.

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Buletinul Academiei de Ştiinţe a Moldovei. Ştiinţele vieţii

Numărul 2(320) / 2013 / ISSN 1857-064X

Rolul condiţiilor de cultivare in biosinteza β-glucanilor la Saccharomyces cerevisiae CNMN-Y-20

Pag. 116-125

Usatîi Agafia, Chiseliţa Natalia, Molodoi Elena, Efremova Nadejda, Bejenaru (Fulga) Ludmila, Borisova Tamara

Institutul de Microbiologie şi Biotehnologie al AŞM

Disponibil în IBN: 18 decembrie 2013

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Sunt expuse rezultatele aprecierii infl uenţei diferitor factori de cultivare asupra tulpinii de levuri Saccharomyces cerevisiae CNMN-Y-20. Articolul include date privind influenţa temperaturii, aeraţiei, duratei de cultivare asupra conţinutului de biomasă, carbohidraţi totali, β-glucani în peretele celular al levurii. Investigaţiile de selectare a condiţilor optime de cultivare dirijată a tulpinii Saccharomyces cerevisiae CNMN-Y-20 au permis elaborarea unui procedeu efi cient de obţinere a β-glucanilor, care poate fi încadrat cu succes în fluxurile tehnologice de producere industrială.

The paper reports the results of the research concerning the influence of various factors on Saccharomyces cerevisiae CNMN-Y-20 strain cultivation. The role of temperature, aeration, рН, yeast cultivation duration in the growth and biosynthesis of polysaccharides, β-glucanes has been defined. The identification of optimum yeast cultivation parameters has resulted in an efficient technique of β-glucane biosynthesis on the Saccharomyces cerevisiae CNMN-Y- 20 strain that can be proposed and employed in the biotechnology of industrial production of glucanes.

Приведены результаты исследований по влиянию различных факторов культивирования штамма Saccharomyces cerevisiae CNMN-Y-20. Определено влияние температуры, аэрации, рН, длительности культивирования дрожжей на рост и биосинтез полисахаридов. Выявленные оптимальные параметры позволили предложить эффективный способ биосинтеза β-глюканов штаммом Saccharomyces cerevisiae CNMNY- 20, который может быть использован в биотехнологии индустриального производства глюканов.

Cuvinte-cheie
Saccharomyces cerevisiae, β-glucani, biomasă celulară, temperatură,

carbohidraţi totali

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