Insufficient scientific information on the quality of walnuts grown in Moldova and the composition of their oils necessitates carry out a systematic study of the most representative varieties of national production in order to contribute with scientific arguments and establish a major potential for walnut oil production at industrial level. The main objectives of the study were to find out solutions in order to obtain walnut oil relatively stable during storage. This paper presents an argumentation of the factors that determine the quality of walnut oil. It presents the chemical composition of oil from walnuts Juglans regia L according to a research conducted in recent years.