Thiosemicarbazide derivatives are widely used in medicine to treat various types of diseases. They all possess a wide range of donor atoms and form with metal ions bio-active coordination compounds of various composition, structure and properties. In this connection, the synthesis and study of new biometal complexes with similar ligands is of both scientific and practical interest.1 The aim of this work is to study the antimicrobial activity of copper (II) coordination compounds containing sulphanilamides (Sf) and 4-phenylthiosemicarbazone pyridine-2-carboxyaldehyde. The composition of the studied compounds is presented in the figure given below. The antimicrobial activity has been studied “in vitro” under liquid nutritive environment (peptonate bullion, pH = 7.0) by means of successive dilutions method. The substances were dissolved in DMSO at a concentration of 10 mM, the subsequent dilutions were prepared under peptonate bullion. The standard stems Staphylococcus aureus, Bacillus cereus, Escherichia coli, Shigela sonnei and Salmonella abony were used as reference culture.It has been established that initial 4-phenylthiosemicarbazone pyridine-2carboxyaldehydes are not active towards the reference culture, whereas the synthesized copper coordination compounds with these ligands display selective bacteriostatic and bactericide activity. The antimicrobial activity towards Gram-positive bacteria is displayed in the concentration range 0.07-75 μg/mL. The minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) of the studied compounds towards Gram-negative test cultures are displayed in the concentration range 0.29-75 μg/mL. The experiment has shown that MIC and MBC of the synthesized compounds are influenced by the nature of inner sphere acid residues and sulphanilamides (Sf): nitrate-, ion-based compounds are more active than chlorine-based complexes. It has been established that depending on the nature of the sulphanilamide the MIC and MBC decrease as follows: Sulphadimezine ≅ Sulphazine > Norsulphazol > Ethazol ≥ Streptocide> Sulphacil.