This study aimed to determine the influence of dandelion polyphenols on the fermentative antioxidant status in the blood serum of breeding roosters, the animals were administered 1 ml of hydro-alcoholic extract for 30 days, with a total polyphenol content of 0.027mg/ml equivalent of gallic acid. For this study, two groups were created: control and experimental, which included 10 animals in each group. As a result of the research we obtained the following values for the control group 110.93±0.30 u/c and for the experimental group 127.5±0.4 u/с, obtaining an increase in superoxide dismutase of approximately 13%, which proves to us that dandelion polyphenols have a stimulating influence on this fermentative index of the antioxidant system. Another link in the antioxidant protection against ROS are the enzymes that inactivate H2O2: catalase and peroxidase. In these studies, values of catalase were obtained for the control group 26.23±0.37 μM/L and correspondingly for the experimental group 29.2±0.82 μM/L, obtaining a difference of approximately 10%. Among the glutathione-dependent enzymes, glutamyltranspeptidase (GTP), and glutathione-S-transferase modifications were investigated. Glutathione is an important regulator of intracellular metabolism. Among the endogenous antioxidants, glutathione is the most important, and in the enzyme system that transforms glutathione, it includes glutathione peroxidase, glutathione-S-transferase, and glutathione reductase. In the conducted research, the following values of GTP were obtained, for the control group 9.33±0.40 u/L, and for the experimental group 10.8±0.66 u/L, which demonstrates an increase in the value of the experimental group by approximately 14%. For glutathione-S-transferase, the value of the control group is 19.21±0.38 nM/sL, and for the experimental group a value of 30.41±0.52 nM/sL was obtained, which constitutes an increase in this index of 36%. Finally, we can conclude that the extract of polyphenols from the dandelion has a stimulating effect on the indices of the fermentative antioxidant system, which in turn influences the physiological and biochemical indices of the organism of breeding roosters through mechanisms of detoxification, regeneration, and maintenance of homeostasis which is very important for the protection from the influence of stressors and other external and internal factors that have a negative influence on the organism.