The researches have been carried out to study the effect of 4-aminobenzoic acid on the growth, lipid synthesis, and fractional composition of lipids in the biomass of Streptomyces massasporeus CNMN-Ac-06 strain. Under laboratory conditions, during cultivation of S. massasporeus CNMN-Ac-06 strain, various concentrations of 4-aminobenzoic acid were added to the SP-I complex medium. The maximum amount of biomass in comparison with the control sample was obtained by cultivation of the strain on SP-I complex medium supplemented with 4-aminobenzoic acid in concentration of 1.37 g/L – 24.99±1.1 g/L, and the amount of total lipids in it was 1.96±0.08 g/L, by 32.28% more in comparison with the control sample. An increase in the phospholipid fraction was obtained on the SP-I complex medium supplemented with 4-aminobenzoic acid in a concentration of 2.74 g/L – 24.39% (when in control sample – 20.70%). Triglyceride and sterol fractions increased on the SP-I complex medium supplemented with 4-aminobenzoic acid in concentration of 1.37 g/L – 18.46% and 20.54%, respectively (when in control sample – 10.55% and 6.80%, respectively).