The paper presents the results of the separate testing of the mineral premix PMVAS, as well as combined with the thermal factor of a moderate stress intensity on saline metabolism in calves in order to determine the parameters that can condition the homeostasis, resistance and adaptive capacities of animals to the influence of the environment. With the separate and combined application of the studied factors in dynamics (7, 30, 60, 90 days) on the organism of the calves, original data were obtained regarding the functional state of the organism. An increase in calcium, phosphorus, potassium, sodium, magnesium and their ratio was found, which denotes a relative restructuring of macroelements’ metabolism in dynamics. The oscillatory character of the values of these elements in the blood of experimental animals reflects on the one hand the amount of macroelements that come into the body through the food ration, and on the other hand the peculiarities of their metabolism. At the same time, we mention that throughout the study period the quantity of macroelements studied in the group in which the food factor was applied in conjunction with the thermal factor is higher than their quantity recorded in the calves from the group in which only the food factor was applied. Thus, a synergistic action of the studied factors is noted, expressed by the potentiation of their effects on the studied macroelements. In the context of the above, we mention that, depending on the application of the factors studied separately or in combination, different results of the saline metabolism indices are obtained, and a moderate intensification of the macroelements’ metabolism in age dynamics, as well as an optimal maintenance of the osmotic balance of the cytosol and extracellular fluid can be noticed in the calves’ body.