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Articolul precedent |
Articolul urmator |
211 8 |
Ultima descărcare din IBN: 2024-03-20 14:26 |
Căutarea după subiecte similare conform CZU |
579.68:556.55(478-25) (1) |
Microbiologie aplicată (368) |
Hidrosfera. Apa în general. Hidrologie (453) |
SM ISO690:2012 BALAN (BATIR), Ludmila, SLANINA, Valerina, BOGDAN-GOLUBI, Nina. Isolation, identification, and conservation of biotechnologically relevant bacteria from the water of “La Izvor” lake. In: One Health and Risk Management, 2023, nr. 4(4), pp. 27-34. ISSN 2587-3458. DOI: https://doi.org/10.38045/ohrm.2023.4.03 |
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One Health and Risk Management | ||||||
Numărul 4(4) / 2023 / ISSN 2587-3458 /ISSNe 2587-3466 | ||||||
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DOI:https://doi.org/10.38045/ohrm.2023.4.03 | ||||||
CZU: 579.68:556.55(478-25) | ||||||
Pag. 27-34 | ||||||
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Introduction. The identification of biotechnologically relevant bacterial strains allows for their utilization as a basis for the development of sustainable agriculture technologies . Material and methods. From the lakes of “La Izvor” Park, 213 representatives of various genera were isolated, out of which 148 were pathogenic. Each suspension culture was diluted until 10-8, serial dilutions were inoculated on plates with Tryptic Soy Agar, Kings medium B, Salmonella Shigella Agar, Kligler's Iron Agar, Endo agar. For the non-pathogenic ones, enzymatic properties were determined, antimicrobial activity was assessed, and they were identified using the Polymerase Chain Reaction (PCR) method. Selected strains were preserved through lyophilization for an extended period. Results. The study revealed significant microbial biodiversity in the lakes of "La Izvor" Park, from which 213 bacterial strains were isolated in pure culture, with 65 selected for further research. The purpose of the research was to isolate and identify non-pathogenic biotechnologically relevant bacteria with various properties that could be applied in the development of sustainable agriculture. Nineteen strains with enhanced enzymatic properties and high antifungal activity were highlighted. These strains were molecularly identified and deposited for inclusion in the National Collection of Non-pathogenic Microorganisms (CNMN). Conclusions. The results of our study have shown that microorganisms can be used as safe biological control agents without the use of chemical compounds, which can prevent soil and water contamination. Lyophilization has confirmed that these strains have tolerance to osmotic and thermal shocks while maintaining viability of over 75%. Based on these properties, the strains are ideal candidates for application in sustainable agriculture |
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Cuvinte-cheie microbial biodiversity, Isolation, molecular identification, long-term conservation, sustainable agriculture, biodiversitate microbiană, izolare, identificare moleculară, conservare îndelungată, agricultură durabilă |
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