The goal of this research was to evaluate the quality of phytomass of the non-native species pearl millet, Pennisetum glaucum, cultivated in the experimental plot of the National Botanical Garden (Institute), Chişinău, Republic of Moldova. It has been found that the green mass contained 116 g/kg CP, 361 g/kg CF, 370 g/kg ADF, 606 g/kg NDF, 33 g/kg ADL, 166 g/kg TSS, 236 g/kg HC, 337 g/kg Cel, with 9.75 MJ/kg ME and 5.58 MJ/kg NEl, the prepared silage was characterized by pleasant smell, pH = 3.78, 18.0 g/kg DM lactic acid and 1.8 g/kg DM acetic acid. The pearl millet grain contained 132.8 g/kg CP, 58.5 g/kg EE, 21 g/kg CF, 764.1 g/kg NFE, 304.6 g/kg starch, 10.9 g/kg ash, 0.6 g/kg Ca, 0.7 g/kg P, 1.09 nutritive units and 11.78 MJ/kg ME, but pearl millet straw 57 g/kg CP, 487 g/kg CF, 530 g/kg ADF, 823 g/kg NDF, 74 g/kg ADL, 293 g/kg HC, 456 g/kg Cel, with 7.92 MJ/kg ME and 3.95 MJ/kg NEl. The pearl millet substrates for anaerobic digestion have C/N = 26-54 with biochemical methane potential 282-375 l/kg. The theoretical ethanol potential from structural carbohydrates of the pearl millet straw averaged 544.4l/t, Pearl millet, Pennisetum glaucum, can be used in many ways: as multi-purpose feed for livestock and as feedstock in the production of renewable energy.