Technology of extraction of herbal bioactive substances using as a clean and safe solvent, supercritical (SC) carbon dioxide (CO2) allows us to obtain, from natural raw materials, highly concentrated extracts in their natural ratio and individual fractions up to the task of obtaining biologically active components for the pharmaceutical cosmetic and food industries [1, 2]. Our proposed technology for producing biologically active extracts by SC-CO2 assumes a high ecological process, since carbon dioxide as a solvent, eliminates the possibility of environmental contamination and the contamination of final product. Moreover, this technology allows to obtain highly concentrated extracts without of residues of any kind of the solvent and saving the biologically active substances in their native proportions. Preservation of biologically active ingredients ensures of a sufficiently low (less than 40 º C) process temperature. Furthermore, the use of CO2 as a solvent at high pressure provides the microbiological purity of the finished product. Our approach to technology development was to study the parameters of the extraction of biologically active substances (phytocomponents): pressure, temperature, solvent consumption, the duration of the supercritical CO2 extracts; to assess the impact of these parameters on the extraction process and content in the extracts of biologically active compounds ( tocoferols, vitamin A, essential fatty acids), with an attempt to develop a kinetic model for the further extraction and establishing optimum conditions of extraction of valuable components for each of the plant materials. The experimental work was carried out using raw materials - wheat germ, (grown in Moldova and Romania). Supercritical CO2 extractions were performed in a laboratory pilot plant equipped with a 1000 cm3 extraction vessel and the two separator vessels of 600 cm3 each, respectively connected in series, with computer software in the process. Analysis of fatty acid composition in the form of methyl esters via trans esterification was carried out on extracts chromatograph Hawlett-Packard HP-6890 capillary column HP c -23. Extraction of wheat germ carried out at 37-42 º C and pressure of 35 -40 MPa in the extractor, with separation of the extract with the parameters: in the first separator at 45 º C and pressure of 14 - 17MPa and at 35 º C, pressure 6,5 - 7,0 MPa in second separator. The optimal duration of extraction was 60 min, the volume passing the extractant - 35 kg on 0.5 kg of raw material.In the development of the parameters evaluated the quality of concentrated extracts of wheat germ and content of biologically active compounds ( tocoferols and vitamin A ), composition of fatty acids in the extract for further justification and standardization of quality indicators. The content of vitamin E (α-γ- δ -tocoferol), depending on the application of the regime ranges: α-tocoferol from 76.16 to 123,6; γ-tocoferol from 43.86 to 67.55, the sum amount of tocoferols from 120.02 to 191.15 mg / 100 g of extract. Contents of vitamin A, extracted under the same parameters i in extracts of the samples were 2900– 3300 IU /1g . Extracts contain mainly bioactive essential polyunsaturated fatty acids, which make up to 64.74% of the total extract (linoleic 18:2 (omega-6), α-linolenic 18:3 ( omega-3), which allows you to use the extract as a dietary supplement for a healthy food, and possibly to create a natural pharmaceuticals AEvit [3]. This work was supported by Academy of Sciences of the Republic of Moldova in cooperation with Research Institute in Romania (project № 24/RoA). We are open for cooperation with research institutes in Russia and Ukraine to the approbation of CO2 extracts. Proposes to establish a data bank of supercritical CO2 extracts of the endemic flora of the Black Sea region (manufacturing, biochemical and clinical assessment of the applicability).