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SM ISO690:2012 DESYATNIK, A., TIURINA, T., GUTSUL, Tatiana, CLAPCO, Steliana, BIVOL, Cezara, NICORICI, Alexandr, RUSU, Emil, LABLYUK, S., DVORNINA, Elena. Effect of nano-oxides of certain metals on biosynthesis of extracellular hydrolases of micromycetes. In: NANO-2016: Ethical, Ecological and Social Problems of Nanoscience and Nanotechnologies, Ed. 2016, 11-14 mai 2016, Chişinău. Chișinău, Republica Moldova: 2016, p. 10. |
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NANO-2016: Ethical, Ecological and Social Problems of Nanoscience and Nanotechnologies 2016 | ||||||
Conferința "NANO-2016: Ethical, Ecological and Social Problems of Nanoscience and Nanotechnologies" 2016, Chişinău, Moldova, 11-14 mai 2016 | ||||||
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To study the effect of nanoparticles on biosynthesis of extracellular hydrolytic |
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Cerif XML Export
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These nanomaterials were<br />characterized by powder X-ray diffraction (XRD), infrared absorption spectroscopy (FTIR)<br />and SEM-microscopy.<br />The assessment of the influence of synthesized nano metal oxides, was carried out<br />according to their impact on biosynthesis of the enzyme at micromycetes Trichoderma<br />koningii, Fusarium gibbosum and Aspergillus niger – producers of extracellular protease and<br />amylase.<br />It was found that the impact of nanomaterials on the synthesis of hydrolases depends<br />on the structure, dimension and concentration of the nanoparticles used as well as on the<br />physiological and biochemical characteristics of the strain and the specificity of action of the<br />enzymes synthesized by them. According to the results of research, ZnO, ZnO/MgO and<br />Fe3O4 nanoparticles may be considered as stimulators of biosynthesis of extracellular<br />proteases in micromycetes Trichoderma koningii and Fusarium gibbosum. However, the<br />degree of their exposure to proteolytic complexes synthesized by two strains was different. In<br />Trichoderma koningii strain this compounds increased the enzyme activity of both acid (by<br />12.1-22.3%) and neutral proteases (by 121.9-188.1%). In Fusarium gibbosum strain the<br />stimulating effect caused by the action of nanoparticles was less significant (by 30.5-40.0%)<br />and occurred only for neutral proteases. For the biosynthesis of amylase synthesized by<br />micromycete Aspergillus niger, the studied a group of nanoparticles had mostly inhibitory<br />effect (by 14.9-54.8%).<br />Biotechnological interest present ZnO nanoparticles of 30nm, which in the<br />concentration of 5-10mg/L in the experimental conditions increased the activity of neutral<br />proteases, as the main component of the enzyme complex strains, up to 3 times.</p></cfAbstr> <cfResPubl_Class> <cfClassId>eda2d9e9-34c5-11e1-b86c-0800200c9a66</cfClassId> <cfClassSchemeId>759af938-34ae-11e1-b86c-0800200c9a66</cfClassSchemeId> <cfStartDate>2016T24:00:00</cfStartDate> </cfResPubl_Class> <cfResPubl_Class> <cfClassId>e601872f-4b7e-4d88-929f-7df027b226c9</cfClassId> <cfClassSchemeId>40e90e2f-446d-460a-98e5-5dce57550c48</cfClassSchemeId> <cfStartDate>2016T24:00:00</cfStartDate> </cfResPubl_Class> <cfPers_ResPubl> <cfPersId>ibn-person-20572</cfPersId> <cfClassId>49815870-1cfe-11e1-8bc2-0800200c9a66</cfClassId> 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