Effect of nano-oxides of certain metals on biosynthesis of extracellular hydrolases of micromycetes
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DESYATNIK, A., TIURINA, T., GUTSUL, Tatiana, CLAPCO, Steliana, BIVOL, Cezara, NICORICI, Alexandr, RUSU, Emil, LABLYUK, S., DVORNINA, Elena. Effect of nano-oxides of certain metals on biosynthesis of extracellular hydrolases of micromycetes. In: NANO-2016: Ethical, Ecological and Social Problems of Nanoscience and Nanotechnologies, Ed. 2016, 11-14 mai 2016, Chişinău. Chișinău, Republica Moldova: 2016, p. 10.
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NANO-2016: Ethical, Ecological and Social Problems of Nanoscience and Nanotechnologies 2016
Conferința "NANO-2016: Ethical, Ecological and Social Problems of Nanoscience and Nanotechnologies"
2016, Chişinău, Moldova, 11-14 mai 2016

Effect of nano-oxides of certain metals on biosynthesis of extracellular hydrolases of micromycetes


Pag. 10-10

Desyatnik A.1, Tiurina T.1, Gutsul Tatiana2, Clapco Steliana1, Bivol Cezara1, Nicorici Alexandr2, Rusu Emil2, Lablyuk S.1, Dvornina Elena1
 
1 Institute of Microbiology and Biotechnology ,
2 Institute of the Electronic Engineering and Nanotechnologies "D. Ghitu"
 
Disponibil în IBN: 27 aprilie 2020


Rezumat

To study the effect of nanoparticles on biosynthesis of extracellular hydrolytic
enzymes metal oxide nanoparticles: MgO, ZnO, ZnO/MgO in a ratio of 1:4, TiO2 and Fe3O4
through hydrothermal and sol-gel techniques were synthesized. These nanomaterials were
characterized by powder X-ray diffraction (XRD), infrared absorption spectroscopy (FTIR)
and SEM-microscopy.
The assessment of the influence of synthesized nano metal oxides, was carried out
according to their impact on biosynthesis of the enzyme at micromycetes Trichoderma
koningii, Fusarium gibbosum and Aspergillus niger – producers of extracellular protease and
amylase.
It was found that the impact of nanomaterials on the synthesis of hydrolases depends
on the structure, dimension and concentration of the nanoparticles used as well as on the
physiological and biochemical characteristics of the strain and the specificity of action of the
enzymes synthesized by them. According to the results of research, ZnO, ZnO/MgO and
Fe3O4 nanoparticles may be considered as stimulators of biosynthesis of extracellular
proteases in micromycetes Trichoderma koningii and Fusarium gibbosum. However, the
degree of their exposure to proteolytic complexes synthesized by two strains was different. In
Trichoderma koningii strain this compounds increased the enzyme activity of both acid (by
12.1-22.3%) and neutral proteases (by 121.9-188.1%). In Fusarium gibbosum strain the
stimulating effect caused by the action of nanoparticles was less significant (by 30.5-40.0%)
and occurred only for neutral proteases. For the biosynthesis of amylase synthesized by
micromycete Aspergillus niger, the studied a group of nanoparticles had mostly inhibitory
effect (by 14.9-54.8%).
Biotechnological interest present ZnO nanoparticles of 30nm, which in the
concentration of 5-10mg/L in the experimental conditions increased the activity of neutral
proteases, as the main component of the enzyme complex strains, up to 3 times.



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<description xml:lang='en' descriptionType='Abstract'><p>To study the effect of nanoparticles on biosynthesis of extracellular hydrolytic<br />enzymes metal oxide nanoparticles: MgO, ZnO, ZnO/MgO in a ratio of 1:4, TiO2 and Fe3O4<br />through hydrothermal and sol-gel techniques were synthesized. These nanomaterials were<br />characterized by powder X-ray diffraction (XRD), infrared absorption spectroscopy (FTIR)<br />and SEM-microscopy.<br />The assessment of the influence of synthesized nano metal oxides, was carried out<br />according to their impact on biosynthesis of the enzyme at micromycetes Trichoderma<br />koningii, Fusarium gibbosum and Aspergillus niger &ndash; producers of extracellular protease and<br />amylase.<br />It was found that the impact of nanomaterials on the synthesis of hydrolases depends<br />on the structure, dimension and concentration of the nanoparticles used as well as on the<br />physiological and biochemical characteristics of the strain and the specificity of action of the<br />enzymes synthesized by them. According to the results of research, ZnO, ZnO/MgO and<br />Fe3O4 nanoparticles may be considered as stimulators of biosynthesis of extracellular<br />proteases in micromycetes Trichoderma koningii and Fusarium gibbosum. However, the<br />degree of their exposure to proteolytic complexes synthesized by two strains was different. In<br />Trichoderma koningii strain this compounds increased the enzyme activity of both acid (by<br />12.1-22.3%) and neutral proteases (by 121.9-188.1%). In Fusarium gibbosum strain the<br />stimulating effect caused by the action of nanoparticles was less significant (by 30.5-40.0%)<br />and occurred only for neutral proteases. For the biosynthesis of amylase synthesized by<br />micromycete Aspergillus niger, the studied a group of nanoparticles had mostly inhibitory<br />effect (by 14.9-54.8%).<br />Biotechnological interest present ZnO nanoparticles of 30nm, which in the<br />concentration of 5-10mg/L in the experimental conditions increased the activity of neutral<br />proteases, as the main component of the enzyme complex strains, up to 3 times.</p></description>
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