There are three main seed-oil crops in Republic of Moldova (rape, sunflower and soybean). These oils are an excellent raw material for biodiesel production. Today the main raw material for obtaining fuel is rock-oil. It should be noted, biodiesel is one of the main alternative fuels for transport systems of the world. For our country biodiesel production has both an ecological side and an aspect of energetic security. By the chemical point of view, biodiesel is a mixture of monoalkyl esters of fatty acids derived from triglycerides through etherification of monohydric alcohols. According to modern technology for biodiesel production, using potassium or sodium hydroxide as a catalyst, glycerol is formed as a by-product, the further utilization of it provokes a lot of problems. Like a way for the problem solution of the utilization of glycerol the Japanese researchers from the Ryukyu University within the framework of the Jatropha Project program, proposed to use a charcoal as a catalyst to biodiesel production from jatropha oil. Using this technology admits to obtain a charcoal-glycerol mixt as by-product , which later can be used like a fertilizer. Researchers from Crop Science Institute "Porumbeni" effected series of experiments to assess the possibilities of charcoal using as catalyst in biodiesel production from rape oil, sunflower oil and soybean oil. The results of the studies showed the biodiesel derived from the above seed oils corresponds completely with the EN14214 standard and can be used as а fuel. Thus, the use of a charcoal as a catalyst for biodiesel production can be used for various oils obtained from the agricultural crops cultivated in Republic of Moldova.