Plant Genetic Resources (PGR) are considered, together with microbial and animal genetic resources, a world heritage, that need to be keep viable for future generation. (Faberova, 2010). The conservation and sustainable utilization of PGR play important role in the improvement and increasing of agriculture productivity, thereby contributing to national development and food security. Every country need to protect especially its original plant materials, such as local genetic resources as advance and old cultivars, breeding materials and crop wild relatives etc. In this context, the documentation of PGR needs a special attention. Documentation refers to completion, analysis, classification, storage and dissemination of information on PGR. This included the development of inventories of genetic resources existing in a country in order to identify gaps and unduplicated materials. Republic of Moldova have been developed own National Inventory (NI) of PGR. The following institutions are involved in PGR activities: Botanical Garden (Institute), Porumbeni Institute of Plant Growing, Practical Scientific Institute of Horticulture and Food Technology (PSIHFT), Institute of Genetics, Physiology and Plant Protection (IGPhPP), Selectia Research Institute of Field Crops. The main activities regarding the protein and oil crops preservation are concentrated especially in the last two institutions. The coordinator of PGR conservation in the country is Laboratory of PGR (IGPhPP) that manages all activities in field of germplasm conservation. In order to strengthen PGR conservation, in this Laboratory has been developed information system ReGen. The main objective of ReGen is to integrate and monitor the available data on existing germplasm collections at the national level. It is important to mention that each collections holder institutions have own information system. However, in the absence of the National Programme for PGR, the interinstitutional collaboration in documentation area is very low. So, the most of information on germplasm collections from holder institutions is not included in ReGen. Only data on small part of the grapevines collection (119 accessions, that constitute 4,8%), fruit trees collections (279 accessions), including apple, apricot, cherry, pears, etc., received from PSIHFT and 310 maize accessions from Porumbeni Institute have been included in ReGen. The analysis of the situation regarding the documentation system at national level allowed to highlight the following constraints on NI: there is no collaboration between all ex situ collections holder institutions and exchange of information practically does not exist; all relevant information on ex situ collections has no documented in the unique database; the lack of common standard format of PGR information; historical gaps in the stored information; insufficient well trained staff in documentation of PGR; insufficient financial resources for maintenance of database. In this context, it is necessary to develop an information-sharing mechanism at the national level, activity that will be managed and monitored by the competent authority on the legal base.