The design and study of well-arranged metal-containing Schiff bases with ONS – donor atoms is an interesting field of inorganic and bioinorganic chemistry. In-situ one pot template condensation reactions lie at the heart of the coordination chemistry. Transition metal complexes have also received a great attention because of their biological interests, including antiviral, anticarcinogenic, antibacterial and antifungal activities. In a recent study, we have concluded that the in vitro HL-60 leukemia cell growth inhibitory activity is unexpected influenced by the nature and geometric structure of copper complexes. Indeed, copper complexes containing tridentate ONS Schiff bases as well as salicylidenthiosemicarbazone have been found effective inhibitors of cell proliferation. We have started a program directed toward the synthesis of different classes of anticancer, antibacterial and antifungal agents designed with complexes of a transition metal and an organic ligand. In continuation of this approach, the present paper describes the synthesis, characterisation and in vitro evaluation of inhibitors of HL-60 cell proliferation, antibacterial and antifungal activity using about thirty novel copper nickel and zinc complexes with the salicylidenthiosemicarbazones (H2 L1 – H2 L10 ) of general formula (X)N-NH-C(S)-NH(Y) obtained from the condensation reaction of thiosemicarbazide (Y = H) or 4-phenylthiosemicarbazide (Y = C6 H5 ) with X = 2-hydroxybenzaldehyde derivatives: OH R R N N H N H S Y OH R R O NH2 N H N H S Y 1 2 1 2 + - H2O The composition and structure of synthesized complexes were analysed by elemental analysis, X-Ray Diffraction, IR and NMR spectroscopies, magnetochemical, thermoanalytical and molar conductance measurements. magnetochemical, thermoanalytical and molar conductance measurements. All ligands and metal complexes were tested as inhibitors of human leukemia (HL-60) cell growth. The most potent Cu (II) complexes, have been also tested for their in vitro antibacterial activity against: a) Grampositive - Staphylococcus aureus (Wood-46, Smith, 209-P), Staphylococcus saprophyticus, Streptococcus (group A), Enterococcus faecalis, Gram-negative - Escherihia coli (O-111), Salmonella typhimurium, Salmonella enteritidis, Klebsiella pneumoniaie, Pseudomonas aeruginosa, Proteus vulgaris and Proteus mirabilis and b) antifungal activity against laboratory stems Aspergillus niger, Aspergillus fumigatus, Candida albicans and Penicillium.