Transnistrian conflict is one of a series of disputes at the EU borders. In the Eastern space, together with the conflict from Eastern Ukraine, Transnistrian dispute worries the European community. Brussels is vitally interested in stability and security in Eastern Europe, because it affects the security and prosperity of EU. Brussels is a member of 5+2 format of negotiations on Transnistrian conflict settlement. Therefore, the EU can contribute to the successful conclusion of negotiations by finding a lasting solution. At the present, the Transnistrian conflict is frozen. Still, because Russia is the supporter of the separatist regime from Tiraspol, in the event of expansion of the conflict from Eastern Ukraine in its Southern region, the Transnistrian conflict could be thawed, i.e. re-launched. This can affect EU as a neighbor of Moldova. Therefore, we can expect that EU will pay sufficient attention to the evolutions inside Moldova and Ukraine. Brussels can have a crucial role in conflict resolution in Transnistria by: 1) monitoring the situation, 2) supporting responsible decision makers, 3) activating a crisis center on Transnistrian conflict and on situation in Republic of Moldova – as an associated to EU state, with serious problems, which affects the European security system.