One of the basic problems of soil microbiology is the regulation of the processes that arise in the soil through the thorough study of their biochemistry, highlighting the role of the groups of microorganisms in carrying out these processes and their action on the process of nutrition in plants. It is well known that nutrient reserves in the form of plant debris accumulate annually in the soil. These reserves can be quickly mineralized by microorganisms and transformed into accessible form for plants. At the same time, the soil contains large quantities of nutrients in various non-accessible forms, which are concentrated in its mineral composition. This is why the use of microorganisms that as a result of their activity contribute to the transformation of inaccessible substances into substances accessible to plants, is a current problem for agriculture, especially for the biological one. On the other hand, it is known that the life span of microbial cells is very short and after their breakdown, they mineralize, forming compounds of nitrogen, phosphorus and other elements easily accessible to plants. The research carried out aims to select and test under laboratory conditions microorganisms with high potential for stimulating plant growth. For this purpose, different cultures of bacteria were isolated from the rhizosphere of the bobo agricultural plants, as a result of which several cultures (strains of rhizoplan, soil, rhizosphere bacteria) were highlighted, growing on different soils of the Republic of Moldova. During the study on the effect of some phytostimulatory microorganisms in the aspect of plant growth and development, the biomass of wheat seedlings increased by 10.5-108.5% on average. In the process of the researches it has been reported that some strains of bacteria positively influence the solubilization of phosphorus from the mineral content of the soil.  It has been found that some strains of bacteria influence the appearance of 3-4 leaves and at the later stages of plant development (maize, wheat, salad). In this case, a particular role belongs to the cell concentration of the bacterial strains used in experiments. The introduction of microorganisms in the soil in the amount of 1: 100 had a considerable contribution to the growth and development of plants. The respective rhizosphere microorganisms sp. (soil), sp.III (nodose), sp. II (rizoplan) selected by the use of bean cutters have revealed another feature of them: to increase both the number of roots and the area occupied by them on the used cuttings. In experiments, concentrations 1/5, 1/100, 1/1000 were used. Therefore, research is carried out on the evaluation of the action of the iron nanoparticles, against the phytostimulatory rhizosphere microorganisms and plants, in the aspect of increasing the efficiency and quality of the soil. The researches carried out under laboratory conditions on some bacteria isolated from the rhizosphere Pseudomonas sp.I, Bacillus sp. II lead to the stimulation of seed germination and plant growth processes, also contributing considerably to the increase of soil quality and fertility.