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Plante aromatice. Condimente. Plante aleaginoase. Plante colorante. Plante tanante. Plante medicinale (433) |
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SM ISO690:2012 DUCA, Maria, GLIJIN, Aliona, ACCIU, Adriana, GISCA, Ion. Markerilor RAPD în screening-ul molecular al rezistenţei florii-soarelui la lupoaie. In: Agronomie şi ecologie, 9-11 octombrie 2013, Chişinău. Chişinău: Centrul editorial UASM, 2013, Vol.39, pp. 168-172. ISBN 978-9975-64-250-7. |
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Agronomie şi ecologie Vol.39, 2013 |
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Simpozionul "Agricultura Modernă – Realizări şi Perspective" Chişinău, Moldova, 9-11 octombrie 2013 | |||||||
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CZU: 633.854.78:631.524.86 | |||||||
Pag. 168-172 | |||||||
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RAPD analysis of different sunflower genotypes was performed using a set of four primers (OPB-10, OPA-11, OPK-17, OPA-02) with arbitrary sequence. Two tested primers (OPB-10 and OPA-2) did not show polymorphism between the included in this study susceptible, tolerant and resistant genotypes. Our results showed a polymorphism between susceptible and resistant sunflower lines in RAPD patterns generated with two of totally four primers used. OPA-11 primer amplified 3 polymorphic bands (570 pb, 850 pb, 960 pb) which were not observed in susceptible and/or tolerant lines. OPK-17 amplified two polymorphic fragments (470 pb and 680 pb) at the some resistant sunflower genotypes. These primers could be used for direct and rapid screening and selection of resistant to Orobanche cumana Wallr. (race E) sunflower lines for the purposes of breeding. |
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Cuvinte-cheie broomrape, molecular markers, RAPD analysis, resistance, Sunflower. |
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