The problem of pesticide degradation in soil is a major current issue of environmental ecology. The most effective destroyers of xenobiotics are microorganisms. The ability of microorganisms to destroy pesticides is related to long-term adaptation to environmental conditions and their activity in many biochemical processes. Numerous studies have shown that greater biodegradation efficiency of organic pesticides takes place under the action of bacterial strains of the genus Pseudomonas, Bacillus, or fungi of the genus Trichoderma, Penicillium, Cladosporium, Aspergillus, and Fusarium. Among the non-pathogenic actinobacteria the genus Rhodococcus are a unique group; they exhibit specific survival skills in critical conditions. They possess the ability to assimilate complex chemical substrates, such as petroleum hydrocarbons, phenolic compounds, humus substances, waxes, resin acids, etc. One important characteristic is their ability to grow and develop in a wide range of concentrations of chemical compounds, which allows surviving in aquatic basins, soils and other polluted areas. There are no available results in this regard on streptomycete strains. Based on the above, the aim of the research was to study the ability of nanoparticles to stimulate the growth of streptomycetes in the presence of trifluralin. Two streptomycetes strains from the National Collection of Non-pathogenic Microorganisms were used in the study: Streptomyces sp. 205 - isolated from soil polluted with pesticides and Streptomyces sp. 12 - isolated from the chernozem of the Central Part of Moldova. There is sufficient data in the literature on the use of nanobiotechnologies to improve the environmental situation, especially for the detection of the active xenobiotic destructive strains. Nanoparticles have been shown to have both stimulatory and inhibitory effect on biomass productivity and composition of various microorganisms: yeasts, fungi, cyanobacteria, etc. Experiments on the action of trifluralin (200 and 300 mg/l concentration) in the presence of Fe0 on the growth of streptomycetes on glucose-free Czapek agar medium demonstrated that the addition of Fe0 nanoparticles at the concentration of 25 mg/l caused changes in the color of the substrate mycelium of Streptomyces sp. 12 from pale yellow to dark red. The appearance of the brown pigment (type 1), the change of chamois color to pale honey and the absence of the 2-type soluble pigment was observed. The following changes were observed in cultural properties of Streptomyces sp. 205 strain: the whitish substrate mycelium became grayish in 1st type colonies; the whitish substrate mycelium became reddish-brown and brownish soluble pigment appeared in 2nd type colonies. Upon addition of 50 mg/l Fe0 of nanoparticles in glucose-free Czapek agar medium, for both strains were detected 3 types of colonies differing by insignificant changes of the aerial mycelium and non-significant changes in the color of substrate mycelium. For Streptomyces sp. 12 the yellow-pale color is changed to the grayish one, and in other two variants the mycelium of the substrate was red-brown and the brown soluble pigment around the colonies with the white and gray aerial mycelium. The highest growth activity of the studied strains was observed at the cultivation of Streptomyces sp. 12 on Czapek medium + 200 mg/l trifluralin (84.25%), and of Streptomyces sp. 205 - on Czapek medium + 200 mg/l trifluralin + 50 mg/l nanoparticles (92.96%). Thus, the conducted studies have shown that the presence of nanoparticles positivelly influence the growth and development of some strains of soil streptomycetes in the medium with trifluralin.