Introduction. One of the challenges for the pharmaceutical industry is the discovery of new pharmacologically molecules, including those of plant origin as antibacterial and antifungal agents. Material and methods. Vegetal product: Agrimoniae herba was harvested from the SPCFMP collection and the extract was obtained with 60% ethanol by maceration and concetration of on the rotative evaporator. The antibacterial and antifungal activities were evaluated by the successive dilution method, which allows the determination of minimum inhibitory concentration and minimum bactericidal concentration of the extract in dilutions:5; 2.5; 1.25; 0.625; 0.312; 0.156; 0.078 mg/ml. Tests were performed on reference strains: Staphylococcus aureus ATCC 25923; Bacillus cereus ATCC 11778; Escherichia coli ATCC 25922; Acinetobacter baumannii ATCC 17978 and the yeast Candida albicans ATCC 10231. Results. Agrimonia eupatoria extract showed bactericidal activity against Gram-positive strains: S. aureus (MIC- 0,156; MBC-0,625) and B. cereus (MIC0.312; MBC-0, 625 mg/ml). Antifungal activity against C. albicans was not shown. Conclusions. Antimicrobial activity of A. eupatoria extract could be related to the content of phenolic compounds as well as their interaction with the susceptibility of the pathogenic microorganism evaluated.