Introduction. Artichoke leaf (Cynarae folium) extracts are known as traditional herbal medicinal products reach in secondary metabolic substances such as: polyphenols, flavonoids, bitter principles and others. The study aimed to investigate in addition the miscellaneous content of artichoke grown in the Republic of Moldova to evince its nutraceutical potential. Material and methods. The leaves of C. scolymus were collected from the SPCFMP of Nicolae Testemitanu SUMPh and subjected to phytochemical analysis through LCHRMS/ MS on an Agilent 1200 HPLC system. The chromatographic separations were carried out with a mobile phase consisting of 0.1% formic acid and 0.1% formic acid in acetonitrile at a flow rate of 0.2 mL/min. Results. A number of seven peaks were characterized as fatty acid derivatives. Four of them were putatively labeled as hydroxylated or oxidized species of octadecatrienoic acid (C18H30O2). The other fatty acids were tentatively assigned as trihydroxyoctadecadienoic acid, trihydroxyoctadecenoic acid and hydroxyoctadecadienoic acid. Conclusions. This study demostrated the presense in Cynarae folium hydromethanolic extracts miscellaneous derivatives which induces an increase in the plant's usage spectrum.