Introduction. Congenital syphilis is a public health problem, that outcomes from an undiagnosed or inadequately addressed case of syphilis in pregnant woman, resulting significant losses of individual health, community costs and social damage. Material and methods. We have made a retrospective medical files analysis of all pregnant women since 1996 till 2022 that were in antenatal care (ANC) in Anenii Noi district. Results. The total of 32778 pregnant women were examined during 1996-2022 period, being constantly more than 95% taken annually in ANC and tested for syphilis at least once during pregnancy. Positive for syphilis were diagnosed in 476 (1,45%) cases. From these positive for syphilis pregnant women a total number of 15 (3,15%) congenital syphilis live cases occurred. During analyzed 1996-2022 period, the average per year distribution of positive for syphilis women were 18,3 cases producing 0,58 cases of live birth cases of congenital syphilis. Making an assumption that all pregnant women produced one livebirth, the overall congenital syphilis rate was 45,72. Conclusion. According to WHO criteria of elimination of mother-to-child transmission of syphilis the main impact indicator is congenital syphilis rate being under 50 referred to 100000 live births. In Anenni Noi district that cumulative indicator for 26 analyzed years proved to be 45,72, being under the threshold, but pretty close to it. The process indicators, as pregnant ANC coverage and testing for syphilis, proved to fit into WHO frames. That case study speaks about validation of mother-to-child transmission of syphilis in Republic of Moldova at district level.