Currently, the most widely used application of sperm preservation is slow freezing, but during this process, sperm undergo cryodestruction, which reduces via-bility and fertility properties. One of the alternatives to slow freezing for spermatozoa is kinetic vitrification, with emerging potential showing encouraging results for vitrification of sperm used in assisted reproduction technologies of the animal kingdom. The application of kinetic vitrification principles is seen as a perspective methodology to different experi-mental models of semen cryopreservation. Kinetic vitrification, in addition to being free of permeable cryoprotectants, includes other advantages such as simplicity, speed, low cost, maintenance of important physiological parameters such as mitochondrial membrane po-tential and DNA integrity. Furthermore, vitrification without cryoprotectants may produce more biological and smaller transverse changes in reproductive cells. Also, the success of cryopreservation is measured by sperm motility after devitrification, where kinetic vitrifi-cation has not yet achieved satisfactory results in experiments performed on the semen of some animals. Thus, in order to elucidate some authentic statements regarding the success of vitrification on the quality of the devitrified semen of different animal species, more stud-ies are needed to obtain sperm viability after devitrification.