The gut microbiota of birds, compared to other animals, is most dependent on living conditions, as they contact with different habitats and intermediate organisms during their lives. Wild birds, due to their lifestyle, diverse feeding habits, ability to migrate long distances, have specific physiological activity which in turn determines the complexity of their gut microbiota. The microbial community in captive birds differs significantly from wild species through differences in environment and diet. Analysis of the intestinal content of bifidobacteria, lactobacteria, enterococci and Escherichia coli in pheasant, black swan and mallard at the Chisinau Zoo revealed the dominance of bifido- and lactobacteria. The numerical indices of intestinal enterococci, which were established in the gut content of investigated birds, indicate a possible symbiosis between them and bifidobacteria and lactobacteria. It can be assumed that this correlation between bifidobacteria and lactobacteria with enterococci keeps the numbers of Escherichia coli at a level that does not allow the development of dysbacteriosis in the wild captive birds. Also, no differences were found in the quantitative levels of bifidobacteria, lactobacteria, enterococci and Escherichia coli according to the phylogeny of the investigated birds and composition of feed. This indicates that the feed rations for wild birds in the Chisinau Zoo are balanced and represent the determining factor in establishing a favorable microbial profile in the gut, which in turn favours the good health of the digestive tract.