Experimental studies by using the spectrophotometric method revealed that lipid peroxidation is species specific. At the same time, the number of diene conjugates in rooster sperm is more than twice than in the bull sperm. The content of this product is not subject to cryogenic changes. The amount of hydroperoxides in rooster sperm is an order of magnitude higher than in bull sperm. It should be noted that cryogenic changes of this indicator are observed only in the bull sperm. While the content of malondialdehyde increased in the sperm of both species in the process of cryopreservation. In the process of technological treatment of sperm of various species of animals, an increase in the content of lipid peroxidation products is caused mainly by malonic dialdehyde, and not to diene conjugates and hydroperoxides, and is also species-specific; the effect of the influence of certain antioxidants on the functional state of gametes depends on the degree of stabilization of intermediate and final products of lipid peroxidation. The conclusion is made about the cryolability of hydroperoxides and malonic dialdehyde, the content of diene conjugates is not affected by cryogenic changes.