Despite advances in the sphere of new drug synthesis, the synthesis of new substances with high biological activity remains an actual problem of modern chemistry. It is caused by widespread dissemination of resistant microorganisms. In recent years coordination compounds of biometals with thiosemicarbazones have been actively researched for solving this problem. However, since thiosemicarbazides containing alkyl or aryl radicals have been used for the synthesis of complexes, it is interesting to find out how the introduction of an unsaturated radical in the composition of thiosemicarbazide affects composition, structure, physicochemical and biological properties of these compounds. Therefore, the aim of this work is the synthesis, determination of the composition, structure, physicochemical and biological properties of chromium, manganese, iron, cobalt, nickel, copper, and zinc coordination compounds with the product of condensation of acetylacetone and 4allylthiosemicarbazone. The experiments showed that chlorides, nitrates, and acetates of stated above metals react with 4-allylthiosemicarbazide and acetylacetone forming solutions of different colors. After cooling of these solutions synthesized coordination compounds MLX2 (M = Cu2+, Ni2+, Mn2+; X = Cl-, NO3 ), M(L-2H) . 2H2O (M = Cu2+, Ni2+, Mn2+), MLX3 ( M = Cr3+, Co3+, X= Cl-, NO3 - ) precipitate. The composition and structure of these compounds was determined on the basis of data from elemental analysis, IR spectroscopy and magnetochemical research. The crystal structure of complex NiL(NO3)2 was determined by X-ray analysis. This complex has a square planar structure. Nitrate ions are in the external sphere. Biological research showed that synthesized coordination compounds show selective bacteriostatic and bactericidal activity. It was shown that the nature of the central atom has a main influence on the antimicrobial activity of these complexes. Depending on the nature of the central atom of homotypic complexes the biological activity diminishes in the following way Cu > Ni > Co ≥ Mn ≥ Cr. The nature of acid residue also has an influence on the activity. For the homotypic complexes it changes in the following way: Cl- > NO3 -. Synthesized compounds manifest the best activity towards the standard strains of gram-pozitive microorganisms (Staphylococcus aureus, Enterococcus faecalis). Their activity towards standard strains of gram-negative microorganisms (Escherichia coli, Salmonella abony) and fungi (Candida albicans) is less pronounced.