Identification of differentially recognized T cell epitopes in the spectrum of tuberculosis infection

Panda Sudhasini; Morgan Jeffrey; Cheng Catherine; Saito Mayuko; Gilman Robert Hugh; Ciobanu Nelly; Krudu V.; Catanzaro Antonino; Catanzaro Donald G.; Rodwell Timothy C.; Perera Judy S. B.; Chathuranga Teshan; Noi Autori

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2024-03-07 09:10

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PANDA, Sudhasini, MORGAN, Jeffrey, CHENG, Catherine, SAITO, Mayuko, GILMAN, Robert Hugh, CIOBANU, Nelly, KRUDU, V., CATANZARO, Antonino, CATANZARO, Donald G., RODWELL, Timothy C., PERERA, Judy S. B., CHATHURANGA, Teshan, NOI, Autori. Identification of differentially recognized T cell epitopes in the spectrum of tuberculosis infection. In: Nature Communications, 2024, vol. 15, pp. 1-14. ISSN 2041-1723. DOI: https://doi.org/10.1038/s41467-024-45058-9

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Nature Communications

Volumul 15 / 2024 / ISSN 2041-1723

Identification of differentially recognized T cell epitopes in the spectrum of tuberculosis infection

DOI:https://doi.org/10.1038/s41467-024-45058-9

Pag. 1-14

Panda Sudhasini¹, Morgan Jeffrey¹, Cheng Catherine¹, Saito Mayuko², Gilman Robert Hugh³, Ciobanu Nelly⁴, Krudu V.⁴, Catanzaro Antonino⁵, Catanzaro Donald G.⁶, Rodwell Timothy C.⁵, Perera Judy S. B.⁷, Chathuranga Teshan⁷, Noi Autori

¹ La Jolla Institute for Immunology,
² Tohoku University ,
³ Peruvian University Cayetano Heredia, Lima,
⁴ Institute of Phtysiopneumology „Chiril Draganiuc”,
⁵ University of California, San Diego,
⁶ University of Arkansas,
⁷ General Sir John Kotelawala Defense University, Ratmalana

Disponibil în IBN: 3 februarie 2024

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There is still incomplete knowledge of which Mycobacterium tuberculosis (Mtb) antigens can trigger distinct T cell responses at different stages of infection. Here, a proteome-wide screen of 20,610 Mtb-derived peptides in 21 patients mid-treatment for active tuberculosis (ATB) reveals IFNγ-specific T cell responses against 137 unique epitopes. Of these, 16% are recognized by two or more participants and predominantly derived from cell wall and cell processes antigens. There is differential recognition of antigens, including TB vaccine candidate antigens, between ATB participants and interferon-gamma release assay (IGRA + /−) individuals. We developed an ATB-specific peptide pool (ATB116) consisting of epitopes exclusively recognized by ATB participants. This pool can distinguish patients with pulmonary ATB from IGRA + /− individuals from various geographical locations, with a sensitivity of over 60% and a specificity exceeding 80%. This proteome-wide screen of T cell reactivity identified infection stage-specific epitopes and antigens for potential use in diagnostics and measuring Mtb-specific immune responses.

Cuvinte-cheie
adult, article, controlled study, Diagnosis, human, immune response, interferon gamma release assay, Male, Mycobacterium tuberculosis, nonhuman, T lymphocyte, tuberculosis