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Articolul urmator |
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SM ISO690:2012 CHESOV, Elena. Corelarea rezistenței genotipice cu cea fenotipică a tulpinilor de Mycobacterium Tuberculosis multidrog rezistente. In: Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”, 21-23 octombrie 2020, Chişinău. Chişinău: USMF, 2020, p. 172. |
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Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu” 2020 | ||||||
Congresul "Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”" Chişinău, Moldova, 21-23 octombrie 2020 | ||||||
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Background. Phenotypic drug susceptibility test (DST) is a recognized reference method for the assessment of drug resistance in M. tuberculosis strains. Feasibility of the replacement of DST by faster whole genome sequencing (WGS) techniques should be investigated. Objective of the study. To assess the degree of correspondence between the culture-based DST and WGS results in multi-drug resistant M. tuberculosis strains from the Republic of Moldova. Material and Methods. We analysed culture based DSTs and compared them with the WGS results in multidrug resistant strains of M. tuberculosis, available in the biobank of the National Reference Laboratory of Mycobacteriology for the years 20172018. Results. Sixty-seven M. tuberculosis strains have been analysed. The genotypic versus phenotypic concordance rate for the resistance to the first line tuberculosis (TB) drugs was: 98,5% in isoniazid, 98,5% - rifampicin, 76,9% - ethambutol and 85,7% - pyrazinamide. In case of second line TB drugs concordance rate was as follow: 94,9% in levofloxacin, 69,6% - ethionamide, 81,3% - amikacin, 65% - capreomycin. Most common mutations associated with TB drug resistance were for isoniazid katG S135T, rifampicin - rpoB S450L, levofloxacin-gyrA D94G and A90V, ethionamide-fabG1 15C>T, amikacin and capreomycin-rss1401a>g. Conclusion. WGS results for M. tubercuslosis strains resistance are comparable with those obtain on culture-based DST. Standardization of laboratory procedures and post-sequencing data interpretation is required for further clinical implementation of WGS. |
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Cuvinte-cheie tuberculosis, resistance, WGS, tuberculoza, rezistenţă, WGS |
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