The Fabaceae species are of great ecological importance due to their capacity to fix biological nitrogen by symbiotic associations, improve the physical properties of soil, form a large amount of organic raw material for various industries and, besides, they are an important source of food and feed protein. Considering the limited material and technical resources in the Republic of Moldova, the efficient use of the biological potential of the perennial leguminous plants that are adapted to the specific climatic conditions becomes more and more relevant. The local ecotype of the species Anthyllis macrocephala, Astragalus cicer, Coronilla varia and Glycyrrhiza glabra maintained in monoculture on experimental land in the NBGI, served as objects of study. After the phenological observations, it was found that, in the first growing season, the growth and development rates of the investigated perennial species was slower in comparison with Medicago sativa. By the end of the growing season, they reached the flowering stage and their green mass could be harvested only once, the green mass yield varied from 0.99 to 1.59 kg/m2, with high content of leaves (68-78 %), it can be used for hay or grazing. In the following years, these species started growing differently; Coronilla varia started 4-6 days earlier than Medicago sativa, but the other species 10-14 days later. The species Anthyllis macrocephala and Coronilla varia were characterized by intensive growth and development rates, which made possible to harvest them in the second half of May as well as Medicago sativa, but Astragalus cicer and Glycyrrhiza glabra reached the stage of flower bud formation in the second half of June, this characteristic would ensure a permanent provision of green fodder for animals. The green mass yield (first mowing) varied from 2.41 to 4.38 kg/m2. 100 kg green mass contained 20- 27 nutritive units, 222293 MJ metabolizable energy, a nutritive unit was provided with 130-144 g digestible protein. It was found that the investigated species were characterized by an optimal content of essential amino acids. Astragalus cicer and Glycyrrhiza glabra fodder was rich in lysine, isoleucine and leucine, but Anthyllis macrocephala contained the same amount of methionine, isoleucine, leucine, valine and threonine as Medicago sativa. The harvested green mass of the studied species can be used as biogas substrate, with carbon nitrogen (C/N) ratios 19 - 23, the gas forming potential reached 501-567 litre/kg VS with 52-53 % methane. The investigated species are an important nectar source for many insects (bees and entomophagous insects) and they are excellent plants to prevent erosion on grass-covered slopes and any areas with loose soil, and for reseeding and increasing the economic value of grasslands.