Sex identification for birds, which are mostly sexually monomorphic (Ong et al., 2008), according to their external morphology is difficult (Griffiths et al., 1998). With the discovery of the CHD gene (chromo-helicase-DNA binding protein, Griffiths and Tiwari 1995) in avian sex chromosome (Ellegren, 1996), molecular DNA noninvasive sexing methods became possible, such as the analysis on feathers and also generated the applicability in the wildlife DNA forensics (An et al., 2006). The avian CHD1 genes belong to a family composed of a chromatin organization modifier (chromo) domain, a SNF2-related helicase/ATPase domain, and a DNA binding domain; thus, the acronym CHD stands for these (Fridolfsson and Ellegren, 1999). CHD genes have in avian genome, also like in other organisms, an important role, being involved in chromatin remodeling in the control of transcription elongation (Simic et al., 2003). This gene is made of two introns with different length for the Z and w chromosome, allowing the discrimination between the products from the Z and W chromosomes on a gel electrophoresis (Dubiec and Zagalska-Neubauer, 2006). Being a functional part of the DNA and having evolved very slowly, the CHD gene is highly conserved, even among distant species. The alignment between CHDW sequence in birds and CHD sequence in mouse doesn’t include any gaps, except two 130 and 175 bp deletions (vucicevic et al., 2012). Its high degree of conservation across species has lead to the design of universal primers for the birds’ sex determination (Griffiths et al., 1998; Khan et al., 1998; Fridolfsson and Ellegren, 1999). The highly conserved domains and the slow evolution, make a gene a good phylogenetic marker, property that CHD genes seems to have, and this is the reason why we ask ourselves: Is the CHD gene a reliable marker for bird populations and phylogenetic analysis? The aim of this study is to test and respond weather the CHD gene is a reliable tool for molecular phylogeny and for population’s analysis of the superfamily sylvioidea. Our result reveal that the CHD genes are good markers for both type of analysis, even better that myoglobin. The implications of the CHD gene in population analysis are high, offering a good image of population dynamics, sex distribution and populations trends. And also, the use of the CHD-Z gene in phylogeny gives the closest to the reality results, having a moderate clock rate and being capable to reveal the relationship between closer and distant taxa.