| SM ISO690:2012|
BRANIŞTE, Tudor; TIGINYANU, Ion; HORVÁTH, Tibor; RAEVSCHI, Simion; GRIDENCO, Oleg; CEBOTARI, Sergiu; HILFIKER, Andres. Guiding of endothelial cells targeted with semiconductor material nanoparticles. In: Health Technology Management3rd International Conference. Editia 3, 6-7 octombrie 2016, Chișinău. Chișinău, Republica Moldova: Technical University of Moldova, 2016, p. 45.
|Health Technology Management
Editia 3, 2016
Conferința "Health Technology Management" |
Chișinău, Moldova, 6-7 octombrie 2016
In this paper we report on the interaction of living endothelial cells (EC) and semiconductor material based nanoparticles (NP). The investigated nanoparticles are based on GaN and Fe2O4Zn. GaN NP with the dimensions ranging from 50 to 100 nm have been obtained in a single HVPE growth process, where GaN layer was deposited on ZnO sacrificial NP. After the growth process ends the ZnO core is being decomposed at high temperatures in H2 flow. Fe2O4Zn NP with the dimensions less than 100 nm have been purchased from Sigma Aldrich. All the experiments have been performed using porcine aorta EC, which have been isolated and labelled as described previously [1,2]. The ECs, passage 6 – 9, were incubated with different doses of floating in the medium NP. It was found that the suitable working concentration of nanoparticles floating in the medium in the process of incubation with EC is less than 100 μg/ml, the higher concentrations affect cells as they diminish the proliferation rate more than 50% comparing with the control group. All the NP are uptaken by the cells within a couple of hours of incubation. We show (see figure 1a) that using a continuous magnetic field it is possible to move targeted cells in a desired manner. In the figure 1b we show the random distribution of the EC after three days of incubation with NP and without any magnetic field influence. The insert in the figure 1b represents the SEM image of EC with uptaken NP. The controlled recellularization represents a great advantage for the further development of tissue engineering applications . Figure 1. Endothelial cells after three days of incubation with Fe2O4Zn nanoparticles. In (a) are presented cells which were exposed to continuous magnetic field and in (b) cells in the control group. The insert from (b) shows the uptaken nanoparticles in the endothelial cells. Conclusions. We found that ECs attract free NPs and, depending on the NP concentration, the cellular activity is slowed down resulting in lower cellular mobility. Nevertheless, cellular proliferation does not seem to be affected as cells continue to divide even when relatively large number of NPs are internalized. Guiding cells targeted with nanoparticles was demonstrated, which represent a step forward to the cellular therapy applications.