Directed synthesis of exopolysaccharides in newly isolated cyanobacteria Nostoc Halphilum Hansg.
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TROFIM, Alina, BULIMAGA, Valentina, ZOSIM, Liliana. Directed synthesis of exopolysaccharides in newly isolated cyanobacteria Nostoc Halphilum Hansg.. In: Microbial Biotechnology, Ed. 4, 11-12 octombrie 2018, Chișinău. Chișinău, Republica Moldova: Institutul de Microbiologie şi Biotehnologie, 2018, Ediția 4, p. 109. ISBN 978-9975-3178-8-7.
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Microbial Biotechnology
Ediția 4, 2018
Conferința "Microbial Biotechnology"
4, Chișinău, Moldova, 11-12 octombrie 2018

Directed synthesis of exopolysaccharides in newly isolated cyanobacteria Nostoc Halphilum Hansg.


Pag. 109-109

Trofim Alina, Bulimaga Valentina, Zosim Liliana
 
Universitatea de Stat din Moldova
 
Disponibil în IBN: 20 februarie 2019



Teza

Exopolysaccharides play a significant biological role in the life of microorganisms, which consume significant amounts of carbon and energy for their production. Exopolysaccharides act as agents of adhesion and facilitate the interactions of cellular associations of microorganisms, including cyanobacteria. Another important function of these substances is the protection from unfavorable physical and chemical factors, or virus and bacteria attacks. Moreover, the exopolysaccharides production presents a way for cyanobacteria to ensure their survival in nutrient-poor environments. Polysaccharides are of great interest to biotechnologists due to the wide range of potential applications in the pharmaceutical, food and cosmetics industries etc., serving as emulsifying agents, as well as, viscosity, dispersion and chelating substances. Investigation of the new potential polysaccharides sources is one of the priorities for modern biotechnology. The purpose of this paper was to investigate the cyanobacterium Nostoc halophilum, as well as the action of some physico-chemical factors on the accumulation of exopolysaccharides. During the investigations of the natural ecosystems, a strain of cyanobacteria that actively vegetates in the soil in the Cogâlnic river meadow Cimislia, R. Moldova was isolated and then obtained a pure culture. This strain is characterized by ribbon trichomes that create dry black crust on the ground. In a pure culture, the strain of cyanobacterium Nostoc halophilum CNMN-CB-17 has been stored in the National Collection of Microorganisms of the Institute of Microbiology and Biotechnology. Cultivation of the strain in two illumination regimes: 1500 lx and from seventh day – 2500 lx with supplementation of 1, 2 and 3 mg/l ammonium acetate revealed that the more intense illumination and the addition of 1 mg/l ammonium acetate led to the highest exopolysaccharide content at the 14th day of cultivation (385 mg/g). With the increase of acetate concentration, the amount of exopolysaccharides was decreasing, reaching values of 179 mg/g at CH3COONH4 concentration of 3 mg/l. Thus, the synthesis of exopolysaccharides by Nostoc halophilum was stimulated by the use of two stress factors: ammonium acetate (1 mg/l) and enhanced illumination (from 1500 to 2500 lx) at the second stage of cultivation.