Migratory birds are often implicated in transporting ticks, which can carry pathogenic agents of several human and domestic animals diseases. By themselves ticks are not highly mobile, and the only ways to expand the habitat are hosts. The present study aimed to evaluate the prevalence and species of exotic ticks infesting wild migratory birds and to assess Borrelia and Rickettsia infection in the collected ticks. During spring we systematically caught and examined migratory birds coming to the Republic Moldova for ticks presence. Migratory birds were caught in ornithological nets at natural reserve Prutul de Jos. All birds were identified to species level, and their sex. Birds were placed in bags made of thick fabric, right after birds were examined in the camp for the presence of ticks. After the examination, birds were released without being harmed. Tick species were detached from birds and stored individually in 70% alcohol. Total DNA was extracted using QIAamp DNA Mini Kit. We screened both ticks and for tick-borne pathogens, including Rickettsia species and Borrelia. Detection of Borrelia burgdorferi s.l. was performed by RT-PCR targeting flaB gene. Rickettsia spp. were detected by PCR targeting the gltA and ompA genes. On proprocession of two spring seasons (2014 and 2015), from the 203 avian specimens collected, 19% of birds (n=39) were infested by ticks (n=82). Ground-feeding birds were commonly infested. Four H. m. marginatum tick specimens were identified. Most collected ticks (93%) were Ixodes ricinus nymphs. Total of 28% of the ticks (n = 23) were positive for infection with B. burgdorferi s.l.. Additionally, two of H. m. marginatum ticks was positive for Rickettsia spp. Hyalomma marginatum ticks have not been registered on the territory of the Republic of Moldova since the 1980s. H. m. marginatum is a tick species usually occurring in relatively dry and warm regions of southern Europe, northern Africa and some parts of Asia. It is a vector of several disease agents of human relevance including Crimean-Congo hemorrhagic fever virus. Reappearance of Hyalomma ticks should be taken seriously and draw further attention to the increasing problem of the import and spread of tropical vectors of disease to Eastern Europe.