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 SM ISO690:2012DUCA, Maria, PORT, Angela, OROZCO-CARDENAS, Martha L., LOVATT, Carol. Transcript analyses for mitochondrial sterile type rearrangement in sunflower. In: Romanian Biotechnological Letters, 2008, vol. 13, p. 0. ISSN 1224-5984. |
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 Romanian Biotechnological Letters |
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| Volumul 13 / 2008 / ISSN 1224-5984 | ||||||
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Transcript analyses were performed to investigate the expression of mitochondrial orfH873 and orf H522 genes present in the rearranged region of PET1. Total RNAs used in cDNA synthesis were isolated from fertile and sterile developing inflorescences of isogenic couple SW501 and fertile restored hybrid Sambred 254 at R1 stage. Using orfH522 sequence as specific primer no transcripts were detected in the fertile line but the male sterile and restored hybrid genotypes investigated showed the same transcript pattern. Results reported here provide the first evidence that other orf from mitochondrial rearranged area - orfH873 is expressed in fertile, sterile and restored hybrid inflorescence buds of sunflower. |
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| Cuvinte-cheie cDNA, Complimentary DNA, Cytoplasmic male sterility, Helianthus annus, orfH 873 |
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