Introduction. The detection of antibioticresistant bacteria results in increased morbidity and mortality among patients with urinary tract infections, as well as length of hospital stay and antibiotic treatment approach. Material and methods. A retrospective analysis of 604 patients with positive urine cultures and 18 strains of microorganisms detected from the MC Sfinta Treime, dept. Nephrology was performed. Results. Laboratory investigations of 634 patients detected 604 urine positive cultures with isolated 1618 microorganisms (48.3%) from bio substrates with positive significance. Gram-negative microorganisms prevails-16 strains (66.6%) and 6 stains gram-positive (33.3%). The gram-positive microorganisms- St. Coagulase-negative -183 (11.3%), St. aureus - 8 (0.5%), St A, B, C, G -16 (0.98) and gram-negative- Enterococcus spp. 460 cases (28.4%), Kl. pneumoniae 277 cases (17.1%), Ps aeroginosa -72 (4.45%), E. coli 482 (29%), candida 170 (10.5%), Proteus spp. 70 (4, 32%). From total of 604 samples, 460 (76.2%) strains were sensitive, 144 samples (23.8%) - resistant to antibiotics. A higher resistance to antibiotics was found in strains of gram-negative microorganisms K. pneumoniae, E. coli, Ps. aeruginosa). Gramnegative microorganisms in 39.12% were resistant. Gram-positive microorganisms showed resistance in 22.20% of the samples and in 77.8% of the samples they were sensitive. Conclusion. A higher resistance to antibiotics was found in strains of gram-negative microorganisms K. pneumoniae, E. coli, Ps. aeruginosa), increased sensitivity to quinolones, carbapenems.