Wound care management therapy represents a major health care problem in the entire world and biomedical research is constantly seeking for new options with respect to promoting the healing process and to reduce the therapy cost. Besides, there are local factors at the wound site that are involved in healing and which consist of moisture, ischemia and/or necrosis of the tissue and existing edema. Many factors including age of the patient, smoking, malnutrition, obesity and chronic diseases, such as diabetes, can delay wound healing and increase the risk of developing chronic wounds. Wound healing still remains problematic and nanotechnology seems to offer the best approach to hasten the healing of acute and chronic wounds. Even though several types of wound dressing materials have already been developed, proper wound healing is far from being easily solved. Electrospun nanofibers (NFs), as novel wound dressings, show two key properties: a high surface/volume ratio and high porosity, properties that are responsible to promote cellular adhesion, proliferation, and differentiation. The design of the NFs offers a wide range of prospects in respect to polymer and active components (ACs) selection. The blends of natural and synthetic polymers are suitable to overcome the low spinnability of the natural polymers. Chitosan/hyaluronic acid-based nanofibers (CS/HA-NFs) have already been described as artificial extracellular matrices (ECMs) due to the resemblance of CS/HA to the glycosaminoglycans of the natural ECMs. CS accelerates the wound healing and the tissue restoration while HA is a non-sulphated glycosaminoglycan, component of the extra cellular matrix.