Obiectivul studiului a fost obținerea extractului uscat de Cynara scolymus L., evaluarea acțiunii antimicotice și formularea a unor supozitoare vaginale cu conținut de extract bogat în compuși fenolici. Extractul uscat, din frunze de C. scolymus colectate din colecția CȘPDPM, a fost obținut la rotavapor. Analiza cantitativă a extractului a fost realizată spectrofotometric, determinând totalul polifenolic și flavonoidic [1]. Supozitoarele au fost formulate utilizând unt de cacao, prin dispersarea extractului cu glicerol-etanol-apa, hidrogel de amidon și alginat de sodiu, cu evaluarea parametrilor farmacotehnici de calitate [2]. Extractul uscat de C. scolymus, cu conținut polifenolic de 54,27±0,02 mg GAE/g și flavonoidic de 18,72±0,4 mg RU/g, a manifestat activitate antifungică față de tulpina Candida albicans ATCC 10231 în diluția de CMI-5mg/ml, CMB-5mg/ml. Supozitoarele vaginale formulate in 3 serii prin fuziune, au fost supuse testelor de calitate, ce au determinat că abaterea de la masa medie (3,07-3,19 g), timpul de topire și de dezagregare, la 36.5±0.5°C în mediu de apă purificată, au corespuns cerințelor farmacopeice. Testul de dizolvare in vitro, a stabilit conținutul totalului de flavonoide cedat (94,57-98,46 %) timp de o oră. Studiul efectuat prezintă o premiză importantă de valorificare a produsului vegetal Cynarae folia, în vederea formulării și condiționării în forme farmaceutice semisolide cu acțiune antimicotică. Cercetările s-au realizat în cadrul proiectului din Programul de Stat 20.80009.8007.24.

The objective of the study was to obtain the dry extract of Cynara scolymus L., evaluating the antifungal action and formulating vaginal suppositories containing plant extract rich in phenolic compounds. The dry extract, from C. scolymus leaves collected from the CȘPDPM collection, was obtained by rotavapor. The quantitative analysis of the extract was carried out spectrophotometrically, determining the total polyphenolic and flavonoid content [1]. The suppositories were formulated using cocoa butter, by dispersing the extract with glycerol-ethanol-water, starch hydrogel and sodium alginate, with the evaluation of the pharmaco-technical quality parameters [2]. The dry extract of C. scolymus, with polyphenolic content of 54.27±0.02 mg GAE/g and flavonoid content of 18.72±0.4 mg RU/g, showed antifungal activity against Candida albicans strain ATCC 10231 in the dilution of CMI-5mg/ml, CMB-5mg/ml. Vaginal suppositories formulated in 3 series by fusion, were subjected to quality tests, which determined that the deviation from the average mass (3.07-3.19 g), melting and disaggregation time, at 36.5±0.5°C in purified water medium, corresponded to the pharmacopoeial requirements. The in vitro dissolution test established the content of total flavonoids release (94.57-98.46 %) during one hour. The carried out study presents an important premise for the usage of Cynarae folia vegetable product, in order to formulate semi-solid pharmaceutical forms with antimycotic action. The research was carried out within the project of the State Program 20.80009.8007.24.

The objective of the study was to obtain the dry extract of Cynara scolymus L., evaluating the antifungal action and formulating vaginal suppositories containing plant extract rich in phenolic compounds. The dry extract, from C. scolymus leaves collected from the CȘPDPM collection, was obtained by rotavapor. The quantitative analysis of the extract was carried out spectrophotometrically, determining the total polyphenolic and flavonoid content [1]. The suppositories were formulated using cocoa butter, by dispersing the extract with glycerol-ethanol-water, starch hydrogel and sodium alginate, with the evaluation of the pharmaco-technical quality parameters [2]. The dry extract of C. scolymus, with polyphenolic content of 54.27±0.02 mg GAE/g and flavonoid content of 18.72±0.4 mg RU/g, showed antifungal activity against Candida albicans strain ATCC 10231 in the dilution of CMI-5mg/ml, CMB-5mg/ml. Vaginal suppositories formulated in 3 series by fusion, were subjected to quality tests, which determined that the deviation from the average mass (3.07-3.19 g), melting and disaggregation time, at 36.5±0.5°C in purified water medium, corresponded to the pharmacopoeial requirements. The in vitro dissolution test established the content of total flavonoids release (94.57-98.46 %) during one hour. The carried out study presents an important premise for the usage of Cynarae folia vegetable product, in order to formulate semi-solid pharmaceutical forms with antimycotic action. The research was carried out within the project of the State Program 20.80009.8007.24.