The autochthonous compound ENOXIL, represents grape seeds tannins with good antioxidant activity, obtained after slow oxidation processes. ENOXIL is, also, known as an efficient and non-toxic medicine against skin and wound infectious agents due to several action mechanisms that it can stimulate: capture of iron ions from the substrate, and depriving microorganisms of compounds necessary for their physiological activity; binding to microbial proteins and formation of complexes; capturing of free radicals; absorption of oxygen radicals; inhibition of low density lipoproteins oxidation. In this study, we investigated the antioxidant activity of ENOXIL by using different free radicals, to betted understand and describe the utility of this compound and its mechanisms of action. DPPH radical and ABTS cation radical can be neutralized via electron or H atom transfer, therefore, these methods have been used to determine the total antioxidant activity of the substrate. The prooxidant activity was tested through Fenton reaction. This method revealed the capacity of ENOXIL to scavenge the aggressive OH radicals. The Folin-Ciocalteu reagent was used due to its selective interaction with phenolic compounds; and the DMACA reagent was utilized due to its reactivity to flavonols.