Soil-borne fungi of the genus Fusarium are among the most harmful due to their ability to produce secondary metabolites - mycotoxins, that make agricultural products dangerous for consumption. The objective of this study was to reveal the presence of mycotoxin-producing sequences of the Fusarium spp. genomes in infected beans using nested-PCR assay. Dry beans of eight Phaseolus vulgaris L. genotypes from the gene bank collection of IGPPP were used in the study (MDC00202, MDC00204, MDC00209, MDC00223, harvest 2015 and 2020). Total DNA was extracted from 0,5 g of seeds according to the CTAB protocols. The PCR was performed in a 25 μl mix as described in the manufacturer's user guide for DreamTaq DNA polymerase (Thermo Fisher Scientific). The first round of the nested-PCR included 950C – 40 sec, 600C – 40 sec, 720C – 40 sec during 30 cycles, final elongation - 7 min, the first cycle denaturation was 950C – 2 min. The second round was performed in the same conditions. Initially DNA samples were tested with the primers designed on the basis of the beta-tubulin gene sequences, which are specific for genus Fusarium. Analysis revealed only one fusarium-bearing sample - MDC 00202, collected in 2020. Further, this sample have been selected for subsequent tests using species-specific primers for identification of F. oxysporum, F. nivale, F. proliferatum, F. verticillioides, F. avenaceum, F. culmorum, F. equiseti, F. sporotrichioides, F. graminearum. As a result of nested-PCR were detected F. sporotrichioides and F. culmorum. These species belong to the group of potential producers of such mycotoxins as Deoxynivalenol (DON), T2-toxin, Zearalenone (ZEN), Fumonisin B1. Subsequently analysis was performed using specific primers for the detection of genes involved in the synthesis of these mycotoxins in the tested sample. For this, a set of primers was used for identification of polyketide synthase (PKS13) genes that are involved in the Zearalenon synthesis, trichothecene biosynthetic gene cluster - DON/T2 synthesis and FUM gene cluster - Fumonisina B1 synthesis. The presence of Fusarium spp. genomic sequences involved in DON/T2 mycotoxin synthesis was revealed.