Among the abiotic stresses that influence plants, drought is one of the most limiting, because it acts directly on the fruit development processes. Under natural conditions, plants at different stages of vegetation, including reproductive ones, are often subjected to the associated action of stress caused by drought and viral pathogens, which can significantly influence the reaction of plants. In relation to this, the purpose of the research was to study the influence of water deficit on the variability of the male gametophyte in tomato offspring, obtained from plants infected with the Tobacco Mosaic Virus (TMV) or Tomato Aspermia Virus (TAV). The reaction of the male gametophyte of the offspring of virus-infected varieties and wild tomato species to the effect of water stress was studied. It has been shown that stress changes the activity of the male gametophyte, reducing the average values of its viability and the length of pollen tubes. Among the genotypes analyzed, the varieties Мary Gratefully and Tomis showed a high level of sensitivity, the viability of their pollen decreased 1.6 ... 1.9 times compared to the control, although in other genotypes the values of this character were more stable. Under conditions of water deficiency, most of the variability was determined mainly by the effect of water deficit and genotype. The analysis of the structure spectrum of variability for each genotype revealed the decisive contribution of water deficit in changing of the pollen tubes length in the majority of TMV/TAV progenies. The variability of pollen viability depending on the genotype was determined by the influence of infection or the effect of water deficit. Analysis of the structure of variation spectra by pollen tube length variability in each genotype established the major contribution of water deficit, although the change in pollen viability according to genotype was determined by water stress or viral agents. In most cases, the average level of resistance at the haploid level in the TMV/TAV descendants was lower than in the control. Based on the established variability, genotypes that possess resistance to water deficit at male gametophyte level were identified.