The studies were performed in 2018-2023 in the ecosystems of Chisinau. The bat fauna of Chisinau is quite well represented, which indicates the adaptation of animals to anthropogenic disturbances in urban ecosystems. Out of 21 species of bats reported on the territory of the Republic of Moldova, 13 species are present in the city ecosystems. The dominant species in the city is Nyctalus noctula, the most synanthropic species that is present in most of old or damaged buildings and even uses the cracks and ventilation pipes of new buildings for roosting. In urban ecosystems, the species N.noctula, Eptesicus serotinus, Pipistrellus pipistrellus/pygmaeus, Pipistrellus austriacus predominate and have been recorded both in green areas and in buildings. The rarest species were Rhinolophus hipposideros, Myotis daubentoni, Myotis dasycneme, Myotis bechsteinii. Most of the registered species are listed in the Red Book of the Republic of Moldova as critically endangered (M. bechsteinii, Vespertilio murinus), endangered (Rh. hipposideros, M. dasycneme, Plecotus auritus) and vulnerable (M. daubentoni, Myotis mystacinus, Pipistrellus kuhli, P. austriacus).