The change of Streptomyces canosus 71 and it’s 2 variants obtained after γ-irradiation antibiotic properties under the influence of combined (γ- and UV-rays) irradiation was studied. The new variants syntesized the secondary metabolites exerted bacteriostatic action on 10 Gram-pozitive and Gram-negative test microorganisms (zones of suppresed growth – 13,0–32,0 mm). Activity to such bacterial agents of bees’ diseases as Bacillus alvea, Bacillus larvae, Streptococcus apis, Salmonella typhimurium was increased noticeable (zones of suppresed growth – 14,0–25,0 mm in compare with 12,014,5 mm for the initial cultures). The variants, obtained after γ- and combined (γ- and UV-rays) irradiation were distinguished by it’s strong selected action on the Candida genus (C. albicans), phytopatogenic bacteria and fungi and agents of bees’ mykoses.