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 SM ISO690:2012RAJABI, Mehdi, SIGNORELLI, Paola, GORINCIOI, Elena, GHIDONI, Riccardo, SANTANIELLO, Enzo. Antiproliferative activity of N6-isopentenyladenosine on MCF-7 breast cancer cells: Cell cycle analysis and DNA-binding study. In: DNA and Cell Biology, 2010, vol. 29, nr. 11, pp. 687-691. ISSN 1044-5498. DOI: https://doi.org/10.1089/dna.2010.1073 |
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  DNA and Cell Biology |
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| Volumul 29, Numărul 11 / 2010 / ISSN 1044-5498 | ||||||
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| DOI:https://doi.org/10.1089/dna.2010.1073 | ||||||
| Pag. 687-691 | ||||||
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N6-Isopentenyladenosine (iPA) is a member of the cytokinins, a family of plant hormones that regulate plant cell growth and differentiation. iPA is present in mammalian cells in a free form, as a mononucleotide in the cytoplasm, or in a tRNA-bound form. Its antiproliferative activity against cancer cells lines has been reported by several authors. We show that iPA inhibits the growth of human breast cancer MCF-7 cells in a dose-dependent manner with a concentration of 12.2μM, which causes 50% inhibition of cell viability. The cell cycle analysis by flow cytometry showed that iPA-induced growth arrest could be associated to apoptosis. The interaction of DNA with iPA was studied in aqueous solution at physiological conditions by ultraviolet-vis spectroscopy. A binding constant KiPA-DNA=4.4×103 M-1 was calculated and this value, together with the shift of absorbance, suggests iPA-DNA interaction at DNA surface. |
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| Cuvinte-cheie absorption, apoptosis, Breast Neoplasms, Cell Cycle, Cell Line, tumor, cell proliferation, DNA, Humans, Isopentenyladenosine, Spectrum analysis, Stereoisomerism |
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