Antiproliferative activity of N6-isopentenyladenosine on MCF-7 breast cancer cells: Cell cycle analysis and DNA-binding study
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RAJABI, Mehdi, SIGNORELLI, Paola, GORINCIOI, Elena, GHIDONI, Riccardo, SANTANIELLO, Enzo. Antiproliferative activity of N6-isopentenyladenosine on MCF-7 breast cancer cells: Cell cycle analysis and DNA-binding study. In: DNA and Cell Biology, 2010, vol. 29, nr. 11, pp. 687-691. ISSN 1044-5498. DOI: https://doi.org/10.1089/dna.2010.1073
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DNA and Cell Biology
Volumul 29, Numărul 11 / 2010 / ISSN 1044-5498

Antiproliferative activity of N6-isopentenyladenosine on MCF-7 breast cancer cells: Cell cycle analysis and DNA-binding study
DOI:https://doi.org/10.1089/dna.2010.1073

Pag. 687-691

Rajabi Mehdi, Signorelli Paola, Gorincioi Elena, Ghidoni Riccardo, Santaniello Enzo
 
University of Milan
 
 
Disponibil în IBN: 15 mai 2023
Rezumat

N6-Isopentenyladenosine (iPA) is a member of the cytokinins, a family of plant hormones that regulate plant cell growth and differentiation. iPA is present in mammalian cells in a free form, as a mononucleotide in the cytoplasm, or in a tRNA-bound form. Its antiproliferative activity against cancer cells lines has been reported by several authors. We show that iPA inhibits the growth of human breast cancer MCF-7 cells in a dose-dependent manner with a concentration of 12.2μM, which causes 50% inhibition of cell viability. The cell cycle analysis by flow cytometry showed that iPA-induced growth arrest could be associated to apoptosis. The interaction of DNA with iPA was studied in aqueous solution at physiological conditions by ultraviolet-vis spectroscopy. A binding constant KiPA-DNA=4.4×103 M-1 was calculated and this value, together with the shift of absorbance, suggests iPA-DNA interaction at DNA surface.
Cuvinte-cheie
absorption, apoptosis, Breast Neoplasms, Cell Cycle, Cell Line, tumor, cell proliferation, DNA, Humans, Isopentenyladenosine, Spectrum analysis, Stereoisomerism

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<cfAbstr cfLangCode='EN' cfTrans='o'><p>N<sup>6</sup>-Isopentenyladenosine (iPA) is a member of the cytokinins, a family of plant hormones that regulate plant cell growth and differentiation. iPA is present in mammalian cells in a free form, as a mononucleotide in the cytoplasm, or in a tRNA-bound form. Its antiproliferative activity against cancer cells lines has been reported by several authors. We show that iPA inhibits the growth of human breast cancer MCF-7 cells in a dose-dependent manner with a concentration of 12.2&mu;M, which causes 50% inhibition of cell viability. The cell cycle analysis by flow cytometry showed that iPA-induced growth arrest could be associated to apoptosis. The interaction of DNA with iPA was studied in aqueous solution at physiological conditions by ultraviolet-vis spectroscopy. A binding constant K<sub>iPA-DNA</sub>=4.4&times;10<sup>3</sup>&nbsp;M<sup>-1</sup>&nbsp;was calculated and this value, together with the shift of absorbance, suggests iPA-DNA interaction at DNA surface.</p></cfAbstr>
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