Conţinutul numărului revistei |
Articolul precedent |
Articolul urmator |
133 0 |
SM ISO690:2012 CHESOV, Dumitru, LANGE, Christoph G., DADUNA, Franziska, KRUDU, V., PREYER, Rosemarie, ERNST, Martin, KALSDORF, Barbara. Combined antigen-specific interferon-γ and interleukin-2 release assay (FluoroSpot) for the diagnosis of Mycobacterium tuberculosis infection. In: PLoS ONE, 2015, vol. 10, nr. 3, pp. 1-14. ISSN 1932-6203. DOI: https://doi.org/10.1371/journal.pone.0120006 |
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PLoS ONE | |
Volumul 10, Numărul 3 / 2015 / ISSN 1932-6203 | |
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DOI:https://doi.org/10.1371/journal.pone.0120006 | |
Pag. 1-14 | |
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Background: To evaluate interleukin (IL)-2 and interferon (IFN)-γ secreting T-cells in parallel for the differentiation of latent infection with Mycobacterium tuberculosis infection (LTBI) from active tuberculosis. Methods: Following ex-vivo stimulation of peripheral blood mononuclear cells (PBMC) with M. tuberculosis-specific antigens early secretory antigenic target (ESAT)-6 and culture filtrate protein (CFP)-10, immune responses were assessed by enzyme-linked immunospot IFN-γ release assay (EliSpot-IGRA) and a novel dual cytokine detecting fluorescence-linked immunospot (FluoroSpot) in 18 patients with pulmonary tuberculosis, 10 persons with previously cured tuberculosis, 25 individuals with LTBI and 16 healthy controls. Results: Correlation of IFN- γ+ spot-forming cells in EliSpot-IGRA and FluoroSpot were R2 = 0.67 for ESAT-6 and R2 = 0.73 for CFP-10. The number of IL-2- IFN- γ+ producing cells was higher in patients with tuberculosis compared with past tuberculosis (CFP-10-induced p = 0.0068) or individuals with LTBI (ESAT-6-induced p = 0.0136). A cutoff value of >16 CFP-10-induced IFN-γ+ secreting cells/200.000 PBMC in the EliSpot-IGRA discriminated with highest sensitivity and specificity (89% and 76%, respectively). However, overlap in cytokine responses precludes distinction between the cohorts on an individual basis. Conclusions: Combined analysis of IFN-γ and IL-2 secretion by antigen specific T-cells does not allow a reliable differentiation between different states of M. tuberculosis infection in clinical practice. |
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Cuvinte-cheie adult, antigens, Bacterial, Bacterial Proteins, Case-Control Studies, female, Humans, immunoassay, Interferon-gamma, Interleukin-2, Male, middle aged, Mycobacterium tuberculosis, tuberculosis |
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