This study aims to investigate the structure and changes in land use from the borders of the Orhei National Park (ONP). ONP is located in the central part of the Republic of Moldova, 90.7% of its surface being located in the Codrilor Plateau and 9.3% in the Dniester Plateau, being extended from west to east over an area of 338.4 km2. In administrative-territorial aspect, ONP overlaps on Orhei, Călăraşi, Străseni and Criuleni districts, 21 settlements are included within them (Vatici, Ivancea, Seliște, Trebujeni, Butuceni, Codreanca etc.). The varied natural conditions, with altitudes ranging from 20.8 m (minimum in Răut meadow) up to 303.1 m (Vârful Dealului hill), that conditioned the formation of a great variety of landscapes. The analysis was based on Landsat 7 and 8 satellite images for 2000 and 2018 years downloaded from https://earthexplorer.usgs.gov/. Six categories of land cover were identified and analyzed: water, arable, forests, grasslands, multiannual plantations and settlements. In order to estimate an optimal land use system, landscape metrics were assessed, which was carried out using the FRAGSTATS and ArcGIS softwares (with the Patch analyst and Patch Grid extensions). In the analyzed time period (2000-2018) qualitative changes have occurred in the landscape structure of the ONP: the share of forests (from 53.9% to 55.8%) and pastures (from 6.6% to 8.5%) increased; the share of arable land (from 31.2% to 28.6%) and multinnual plantations (from 3.3% to 2.3%) decreased. The water landscapes remained practically unchanged. The increase in the average size of elementary landscape units during the analyzed period contributed to reducing the degree of fragmentation, which has a positive impact on the environment. The use of landscape metrics offers many facilities in studies of landscape ecology and recommendations to improve the landscapes state.