A Saccharomyces cerevisiae strain, isolated from “Fetească regală” grapes cultivated in “Pietroasa” Viticulture and Winemaking Research and Development Center, belonging to the University of Agronomic Sciences and Veterinary Medicine of Bucharest (USAMV) and identified by molecular sequencing, was used for cultivation on a synthetic medium to obtain active dry yeast biomass. The scale-up from laboratory level at micropilot level was performed in this study, following the optimization of the process at laboratory level. The pre-inoculum was based on pure culture of Saccharomyces cerevisiae which was used to inoculate a liquid YSP medium (yeast extract, hy-soy peptone, and sucrose) to obtain the liquid inoculum. The liquid inoculum was used to inoculate the fermentation medium (carbon source - sugar and nitrogen source - yeast extract) from the bioreactor (working volume 4L). The cultivation parameters were monitored online (temperature 30ᵒC, stirring rate 200-300 rpm, pH 4-5) and off line (total soluble dry matter, pH; wet cell biomass and dry cell biomass). Finally, following the post-fermentation process, a wet biomass of 46.5 g/L was obtained, respectively active dry wine yeast 14.79 g/L, which will be used in the experiments regarding the production of “Fetească regală” wines from “Pietroasa” Viticulture and Winemaking Research and Development Center, USAMV, Bucharest for assessing the terroir behavior. Acknowledgements: This work was supported by a grant of the Ministry of Research, Innovation and Digitization, CNCS/CCCDI-UEFISCDI, project number PN-III-P3-3.5- EUK-2019-0213, within PNCDI III