The use of qNMR spectroscopy for analytical evaluation of lavender extracts. Determination of rosmarinic acid
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543.429.23:633.812 (1)
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BÎRCA, Natalia, BARBA, Alic, KULCIŢKI, Veaceslav. The use of qNMR spectroscopy for analytical evaluation of lavender extracts. Determination of rosmarinic acid. In: Life sciences in the dialogue of generations: connections between universities, academia and business community, Ed. 2, 29-30 septembrie 2022, Chişinău. Chișinău, Republica Moldova: Moldova State University, 2022, p. 203. ISBN 978-9975-159-80-7.
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Life sciences in the dialogue of generations: connections between universities, academia and business community 2022
Conferința "Life sciences in the dialogue of generations: connections between universities, academia and business community"
2, Chişinău, Moldova, 29-30 septembrie 2022

The use of qNMR spectroscopy for analytical evaluation of lavender extracts. Determination of rosmarinic acid

CZU: 543.429.23:633.812

Pag. 203-203

Bîrca Natalia, Barba Alic, Kulciţki Veaceslav
 
Institute of Chemistry
 
Proiect:
20.80009.8007.03 Noi substanțe cu potențial preventiv și terapeutic în baza compușilor naturali de origine vegetală și a matodelor moderne de sinteză organică
 
Disponibil în IBN: 24 noiembrie 2022


Rezumat

NMR spectroscopy is a powerful method for structural identification of both individual compounds and complex natural products mixtures. The main advantages of this modern analytical tool are, among others, high resolution power, as well as simultaneous qualitative and quantitative evaluation of the investigated sample [1]. The current work presents the determination of rosmarinic acid (RA) content in lavender extracts basing on fast and reliable 2D NMR correlations. Few data on the quantitative determination of RA in lavender are known in the literature, even though it is an abundant and highly valuable secondary metabolite [2]. A pure sample of RA was isolated from dried leaves of lemon balm (Melissa officinalis L.) and its 1H and HSQC spectra recorded. Purity grade of the obtained sample was calculated basing on 1H NMR and internal standard (IS) method. Methyl 4-nitrobenzoate was used as an IS, both for 1H and HSQC measurements. Two diagnostic signals of RA have been chosen on the basis of their separation from the IS peaks. The HSQC relaxation delay parameter (D1) was optimized basing on the relaxation times T1 determined for both diagnostic signals. The calibration curve was drawn on the basis of HSQC correlation, plotting the peak volumes of the chosen diagnostic peak, normalized to the peak volume of IS versus molar ratio between RA and IS. A perfect linearity was achieved. The quantitative determination of RA in a lavender extract was easily performed, thanks to an excellent resolution of RA diagnostic signals from all other extract components cross peaks. The elaborated method can be routinely used for the quality control of herbal pharmaceuticals or related products. The analysis time is comparable with the time of an average HPLC experiment. The sample preparation procedure and interpretation of results are very simple and can be easily adapted for any modern analytical laboratory.