Aim: Spectrophotometric determination of total hydroxycinnamic acids in the aerial parts of Agrimoniae herba (Agrimonia eupatoria L., fam. Rosaceae) and Cichorii herba (Cichorium intybus L., fam. Asteraceae). Methodology: The aerial parts of agrimony and chicory were harvested in the flowering period from the collection of Scientific Practical Centre in the Field of Medicinal Plants (SPCFMP) of “Nicolae Testemițanu” State University of Medicine and Pharmacy, during the flowering period (2021). The dry extracts have been obtained through a fractional maceration method and afterwards have been concentrated using the Laborota 4011 rotative evaporator. The quantitative analysis of total hydroxycinnamic acids (THA) was done by three methods using a Metertech UV/VIS SP 8001 spectrophotometer. Results: The spectrophotometric analysis performed with Arnow reagent (518 nm), according to the European Pharmacopoeia, determined the total hydroxycinnamic acids content (caffeic acid equivalents): 3.67 mg/g for A. eupatoria and 1.48 mg/g for C. intybus. Our results showed a higher content of total hydroxycinnamic acids in chicory in the extractive solution obtained with 30% ethanol: 13.22% (chlorogenic acid equivalents) than in 20% ethanol extractive solution: 10.93% (caffeic acid equivalents), followed by agrimony (3.78% and 3.10%, respectively). In the last two methods, the optical density of the extracts was read at 325 nm. Conclusions: The total content of hydroxycinnamic acids was determined by three spectrophotometric methods. Extracts of the aerial parts of A. eupatoria and C. intybus species from the collection of SPCFMP can serve as sources of phenolic acids.