The milk productivity of sheep raised in Moldova is not high. At the Tsigay breed of sheep, the average productivity per lactation is 105–120 liters and at the Karakul breed is 65–80 liters. For improving the milk productivity at the Tsigay sheep, in 2014 dairy sheep of the Assaf breed were imported, whose homeland is Israel, and are mainly bred in Spain, where the climate is the same as in the southern part of Moldova, the Budjak steppe. The aim of the research was to study, in the natural and climatic conditions of the Republic of Moldova, at ewes of local reproduction, the general development in terms of physique indices, the milk productivity, the study of udder measurements and the effect of the lactation number of ewes on milk production in sucking, milking periods and in general for lactation, as well as the chemical composition of milk. From the calculated 6 body build indices at ewes of four years of age, the indicators are lower by four indices in comparison with two and three years of age. In comparison with ewes of two years of age: on the transverse by 1.7%; massiveness by 2.1%; for breastfeeding by 6.5% and consistency by 5.6%, and in comparison with ewes of three years of age, respectively, by: 2.7%; 1.5%; 6.0% and 1.5%. On the second-third month of lactation, the development of the mammary gland was studied on 5 ewes at two, three and four years, which corresponds to I, II and III lactations. Using the obtained data on measurements of the udder itself, the main indicator of its volume was calculated. The highest at ewes in the third lactation is 3717 cm3, in comparison with ewes of the first lactation, it is higher by 986 cm3 (P≤0.01) and by 243 cm3 higher in relation to ewes of the second lactation. The length and diameter of the teats correspond to the requirements for suitability for machine milking. Milk productivity for the first 20 days after lambing is the highest 55.96±2.07 liters at ewes for the third lactation. At ewes for the first lactation it is less by 20.74 liters (P≤0.001) and for the second by 16.06 liters (P≤0.05) in relation to ewes for the third lactation. During the suckling period at ewes for the third lactation, milk production, as in the first 20 days after lambing, is the highest 240.94 liters. In relation to the first lactation it is higher by 49.22 liters and to the second lactation by 34.14 liters. The duration of the milking period is 135 days for which at ewes for first lactation was yielded 204.08 liters, for the second lactation 219.12 and for ewes of the third lactation 215.25 liters. In general, for lactation, 456.38 liters of milk was received from ewes for the third lactation, or by 60.58 liters more in comparison with ewes of the first lactation and by 30.45 liters from ewes of the second lactation. In all studied periods, at ewes on the third lactation, the indicators are better in comparison with the indicators of the first and second lactations. For studying the chemical composition of milk from the ewes of the studied groups, milk samples were taken and using the Lactosan MCC device were determined the amount of fat, protein, lactose, DSMR, salts and its density. At ewes for the third lactation, the indicators are higher in comparison with ewes for the first and second lactations. Based on the data obtained, it can be concluded that that sheep of the specialized dairy breed Assaf have acclimatized to the conditions of growing in the Republic of Moldova and can be successfully used by sheep breeders.